Evaluation of histochemical and molecular markers in the placenta of sows during pregnancy complicated by isoimmunization

It is known that the pathological course of pregnancy associated with a disturbance in interdependent “mother-fetus” complex and leads to specific clinical consequences at molecular-cellular, tissue, organ, organismal population levels organization. In case violation placentation (placental barrier), state immune conflict occurs, characterized by an antigen-antibody reaction, carried out through placenta (in relation fetus), or colostrum after birth newborn offspring). Therefore, immunological relations level should be considered both action maternal organism’s antigens on fetus, effects allogeneic fetus mother’s body. According results experiment, placentas 10 sows Large White breed were used as material, which showed signs isoimmunization resulting offspring. For histological examination, immediately delivery, pieces up 0.5 cm thick selected, fixed 10% aqueous solution neutral formalin. The material wiring alcohols increasing concentration, xylene, xylene-paraffin, was poured into paraffin. obtained preparations stained conventional methods-hematoxylin eosin. d (fibrinoid masses).